气候变化领域集成服务门户(CCPortal): The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t

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DOI	10.1094/MPMI-22-4-0411
	The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t
	Li, Wei; Wang, Baohua; Wu, Jun; Lu, Guodong; Hu, Yajun; Zhang, Xing; Zhang, Zhengguang; Zhao, Qiang; Feng, Qi; Zhang, Hongyan; Wang, Zhengyi; Wang, GuoLiang; Han, Bin; Wang, Zonghua; Zhou, Bo
发表日期	2009
ISSN	0894-0282
EISSN	1943-7706
卷号	22 期号:4
英文摘要	The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae.
学科领域	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
来源期刊	MOLECULAR PLANT-MICROBE INTERACTIONS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111564
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Gene Res, Shanghai 200233, Peoples R China
推荐引用方式 GB/T 7714	Li, Wei,Wang, Baohua,Wu, Jun,et al. The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t[J]. 中国科学院西北生态环境资源研究院,2009,22(4).
APA	Li, Wei.,Wang, Baohua.,Wu, Jun.,Lu, Guodong.,Hu, Yajun.,...&Zhou, Bo.(2009).The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t.MOLECULAR PLANT-MICROBE INTERACTIONS,22(4).
MLA	Li, Wei,et al."The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t".MOLECULAR PLANT-MICROBE INTERACTIONS 22.4(2009).