气候变化领域集成服务门户(CCPortal): Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function

CCPortal

DOI	10.1089/cbr.2008.0514
	Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function
	Liu, Bing; Zhang, Hong; Duan, Xin; Hao, Pang; Xie, Yi; Zhou, Qingming; Wang, Yanling; Tian, Yuan; Wang, Tao
发表日期	2009
ISSN	1084-9785
EISSN	1557-8852
卷号	24 期号:1
英文摘要	To determine whether adenovirus-mediated wild-type p53 transfer after radiotherapy Could radiosensitize non-small-cell lung cancer (NSCLC) cells to subclinical-dose carbon-ion beam (C-beam), H1299 cells were exposed to a C-beam or gamma-ray and then infected with 5 MOI of AdCMV-p53 or GFP (C-beam or gamma-ray with p53 or GFP). Cell cycle was detected by flow cytometric analysis. The apoptosis was examined by a fluorescent microscope with DAPI staining. DNA fragmentation was monitored by the TUNEL assay. P53 mRNA was detected by reverse-transcriptase polymerase chain reaction. The expression of p53, MDM2, and p21 was monitored by Western blot. Survival fractions were determined by colony-forming assay. The percentages of G(1)-phase cells in C-beam with p53 increased by 8.2%-16.0%, 5.2%-7.0%, and 5.8%-18.9%, respectively, compared with C-beam only, gamma-ray with p53, or p53 only. The accumulation of G(2)-phase cells in C-beam with p53 increased by 5.7%-8.9% and 8.8%-14.8%, compared with those in gamma-ray with p53 or p53 only, respectively. The percentage of apoptosis for C-beam with p53 increased by 7.4%-19.1%, 5.8%-11.7%, and 5.2%-19.2%, respectively, compared with C-beam only, gamma-ray with p53, or p53 only. The level of p53 mRNA in C-beam with p53 was significantly higher than that in p53 only. The expression level of p53 and p21 in C-beam with p53 was significantly higher than that in both C-beam with GFP and p53 only. The survival fractions for C-beam with p53 were significantly less than those for the other groups (p < 0.05). The data suggested that AdCMV-p53 transfer could more efficiently radiosensitize H1299 cells to subclinical-dose C-beam irradiation through the restoration of p53 function.
关键词	carbon-ion beam subclinical-dose p53 gene transfer non-small-cell lung cancer adenovirus vector radiosensitivity
学科领域	Oncology; Research & Experimental Medicine; Pharmacology & Pharmacy; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
WOS研究方向	Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
来源期刊	CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111551
作者单位	Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 73000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bing,Zhang, Hong,Duan, Xin,et al. Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function[J]. 中国科学院西北生态环境资源研究院,2009,24(1).
APA	Liu, Bing.,Zhang, Hong.,Duan, Xin.,Hao, Pang.,Xie, Yi.,...&Wang, Tao.(2009).Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,24(1).
MLA	Liu, Bing,et al."Adenovirus-Mediated Wild-Type p53 Transfer Radiosensitizes H1299 Cells to Subclinical-Dose Carbon-Ion Irradiation Through the Restoration of p53 Function".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 24.1(2009).