Climate Change Data Portal
DOI | 10.1002/bit.27149 |
Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation | |
Sung, Young Joon; Choi, Hong Il; Lee, Jeong Seop; Hong, Min Eui; Sim, Sang Jun | |
发表日期 | 2019 |
ISSN | 0006-3592 |
EISSN | 1097-0290 |
英文摘要 | Microalgae have a high potential to be utilized as feedstock for biofuels because they have high growth rates and do not compromise food production. Commercialized algae-based biofuel production relies on the development of strains with high lipid content. Based on the relatively low density of lipids compared to other cellular components, density gradient centrifugation was used to isolate high lipid content algal strains from Chlamydomonas reinhardtii mutant libraries. The correlation between cell density and lipid content was confirmed by analysis of Nile red fluorescence intensity, total lipids, and total fatty acid methyl ester content. A strain isolated by this screening method had 50% higher lipid content and 7% lower cell density than the parent wild-type strain. Consequently, we demonstrated that screening of algal strains with low cell density via continuous density gradient centrifugation allows simple, rapid, and inexpensive screening for high lipid content strains. |
WOS研究方向 | Biotechnology & Applied Microbiology |
来源期刊 | BIOTECHNOLOGY AND BIOENGINEERING
![]() |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/102714 |
作者单位 | Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea |
推荐引用方式 GB/T 7714 | Sung, Young Joon,Choi, Hong Il,Lee, Jeong Seop,et al. Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation[J],2019. |
APA | Sung, Young Joon,Choi, Hong Il,Lee, Jeong Seop,Hong, Min Eui,&Sim, Sang Jun.(2019).Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation.BIOTECHNOLOGY AND BIOENGINEERING. |
MLA | Sung, Young Joon,et al."Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation".BIOTECHNOLOGY AND BIOENGINEERING (2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。