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DOI | 10.1016/j.jprot.2019.103396 |
Environmental temperature variation affects brain protein expression and cognitive abilities in adult zebrafish (Danio rerio): A proteomic and behavioural study | |
Toni, M.1; Angiulli, E.1; Miccoli, G.1; Cioni, C.1; Alleva, E.3; Frabetti, F.4; Pizzetti, F.4; Scalvini, F. Grassi2; Nonnis, S.2; Negri, A.2; Tedeschi, G.2; Maffioli, E.2 | |
发表日期 | 2019 |
ISSN | 1874-3919 |
EISSN | 1876-7737 |
卷号 | 204 |
英文摘要 | Water temperature is an important environmental parameter influencing the distribution and the health of fishes and it plays a central role in ectothermic animals. The aim of this study is to determine the effects of environmental temperature on the brain proteome and the behavioural responses in zebrafish, a widely used animal model for environmental "omics" studies. Adult specimens of wild-type zebrafish were kept at 18 degrees C, 34 degrees C and 26 degrees C (control) for 21 days. Proteomic data revealed that several proteins involved in cytoskeletal organization, mitochondrial regulation and energy metabolism are differently regulated at the extreme temperatures. In particular, the expression of proteins associated to synapses and neurotransmitter release is down-regulated at 18 degrees C and 34 degrees C. In both thermal conditions, fish exhibited a reduced interest for the novel environment and an impairment of cognitive abilities during Y-Maze behavioural tests. The observed pathways of protein expression are possibly associated to functional alterations of the synaptic transmission that may result in cognitive functions impairment at central nervous system level as those revealed by behavioural tests. This study indicates that temperature variations can elicit biochemical changes that may affect fish health and behaviour. This combined approach provides insights into mechanisms supporting thermal acclimation and plasticity in fishes. Significance: Environmental temperature variation may impact on all levels of biological life. Understanding the impact of thermal variation on the nervous system and animal behaviour is of primary importance since the results obtained can be applied from the ecological to the biomedical fields. |
WOS研究方向 | Biochemistry & Molecular Biology |
来源期刊 | JOURNAL OF PROTEOMICS |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/100824 |
作者单位 | 1.Sapienza Univ, Dept Biol & Biotechnol Charles Darwin, Rome, Italy; 2.Univ Milan, Dept Vet Med, Milan, Italy; 3.Ist Super Sanita, Ctr Behav Sci & Mental Hlth, Rome, Italy; 4.Univ Bologna, Dept Expt Diagnost & Specialty Med, Bologna, Italy |
推荐引用方式 GB/T 7714 | Toni, M.,Angiulli, E.,Miccoli, G.,et al. Environmental temperature variation affects brain protein expression and cognitive abilities in adult zebrafish (Danio rerio): A proteomic and behavioural study[J],2019,204. |
APA | Toni, M..,Angiulli, E..,Miccoli, G..,Cioni, C..,Alleva, E..,...&Maffioli, E..(2019).Environmental temperature variation affects brain protein expression and cognitive abilities in adult zebrafish (Danio rerio): A proteomic and behavioural study.JOURNAL OF PROTEOMICS,204. |
MLA | Toni, M.,et al."Environmental temperature variation affects brain protein expression and cognitive abilities in adult zebrafish (Danio rerio): A proteomic and behavioural study".JOURNAL OF PROTEOMICS 204(2019). |
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