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DOI | 10.3389/fmicb.2019.01656 |
Contrasting Winter Versus Summer Microbial Communities and Metabolic Functions in a Permafrost Thaw Lake | |
Vigneron, Adrien1,2,3; Lovejoy, Connie1,2,3,4; Cruaud, Perrine3,5; Kalenitchenko, Dimitri1,3,4; Culley, Alexander2,3,5; Vincent, Warwick F.1,2,3 | |
发表日期 | 2019 |
ISSN | 1664-302X |
卷号 | 10 |
英文摘要 | Permafrost thawing results in the formation of thermokarst lakes, which are biogeochemical hotspots in northern landscapes and strong emitters of greenhouse gasses to the atmosphere. Most studies of thermokarst lakes have been in summer, despite the predominance of winter and ice-cover over much of the year, and the microbial ecology of these waters under ice remains poorly understood. Here we first compared the summer versus winter microbiomes of a subarctic thermokarst lake using DNA-and RNA-based 16S rRNA amplicon sequencing and qPCR. We then applied comparative metagenomics and used genomic bin reconstruction to compare the two seasons for changes in potential metabolic functions in the thermokarst lake microbiome. In summer, the microbial community was dominated by Actinobacteria and Betaproteobacteria, with phototrophic and aerobic pathways consistent with the utilization of labile and photodegraded substrates. The microbial community was strikingly different in winter, with dominance of methanogens, Planctomycetes, Chloroflexi and Deltaproteobacteria, along with various taxa of the Patescibacteria/Candidate Phyla Radiation (Parcubacteria, Microgenomates, Omnitrophica, Aminicenantes). The latter group was underestimated or absent in the amplicon survey, but accounted for about a third of the metagenomic reads. The winter lineages were associated with multiple reductive metabolic processes, fermentations and pathways for the mobilization and degradation of complex organic matter, along with a strong potential for syntrophy or cross-feeding. The results imply that the summer community represents a transient stage of the annual cycle, and that carbon dioxide and methane production continue through the prolonged season of ice cover via a taxonomically distinct winter community and diverse mechanisms of permafrost carbon transformation. |
WOS研究方向 | Microbiology |
来源期刊 | FRONTIERS IN MICROBIOLOGY |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/100560 |
作者单位 | 1.Univ Laval, Dept Biol, Quebec City, PQ, Canada; 2.Univ Laval, Ctr Etud Nord, Takuvik Joint Int Lab, Quebec City, PQ, Canada; 3.Univ Laval, Inst Biol Integrat & Syst, Quebec City, PQ, Canada; 4.Univ Laval, Quebec Ocean, Quebec City, PQ, Canada; 5.Univ Laval, Dept Biochim Microbiol & Bioinformat, Quebec City, PQ, Canada |
推荐引用方式 GB/T 7714 | Vigneron, Adrien,Lovejoy, Connie,Cruaud, Perrine,et al. Contrasting Winter Versus Summer Microbial Communities and Metabolic Functions in a Permafrost Thaw Lake[J],2019,10. |
APA | Vigneron, Adrien,Lovejoy, Connie,Cruaud, Perrine,Kalenitchenko, Dimitri,Culley, Alexander,&Vincent, Warwick F..(2019).Contrasting Winter Versus Summer Microbial Communities and Metabolic Functions in a Permafrost Thaw Lake.FRONTIERS IN MICROBIOLOGY,10. |
MLA | Vigneron, Adrien,et al."Contrasting Winter Versus Summer Microbial Communities and Metabolic Functions in a Permafrost Thaw Lake".FRONTIERS IN MICROBIOLOGY 10(2019). |
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