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DOI | 10.3389/fevo.2019.00260 |
How Does eDNA Compare to Traditional Trapping? Detecting Mosquito Communities in South-African Freshwater Ponds | |
Krol, Louie1,2; Van der Hoorn, Berry2; Gorsich, Erin E.3,4; Trimbos, Krijn1; van Bodegom, Peter M.1; Schrama, Maarten1,2 | |
发表日期 | 2019 |
ISSN | 2296-701X |
卷号 | 7 |
英文摘要 | Improved biomonitoring of mosquitoes requires an in-depth understanding on occurrences of both vector and non-vector species, in larval, and adult stages. Accurate descriptions of the ecological context in which mosquitoes thrive remain limited, particularly for larval stages. The aim of this study was to develop amixed-amplicon eDNA approach to assess (i) whether mosquito larval communities of stagnant fresh-water bodies can be detected using a Culicidae-specific primer and (ii) how these results compare to traditional trapping of adult mosquitoes. Results from 32 ponds inside and outside Kruger National Park, South Africa show that our primer detected mosquito eDNA. However, it yielded only a subset of the species found using adult trapping methods. Particularly the less frequent and container-breeding species were not found. Our approach provides the first steps toward an eDNA-based method to assess the entire community of larval-stage mosquitoes. It may thereby overcome current taxonomic hurdles presented by morphological identification of larvae. As such, it holds great promise for biomonitoring and ecological studies of mosquitoes. |
WOS研究方向 | Environmental Sciences & Ecology |
来源期刊 | FRONTIERS IN ECOLOGY AND EVOLUTION
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/100392 |
作者单位 | 1.Leiden Univ, Inst Environm Sci, Leiden, Netherlands; 2.Nat Biodivers Ctr, Leiden, Netherlands; 3.Univ Warwick, Zeeman Inst Syst Biol & Infect Dis Epidemiol Res, Coventry, W Midlands, England; 4.Univ Warwick, Sch Life Sci, Coventry, W Midlands, England |
推荐引用方式 GB/T 7714 | Krol, Louie,Van der Hoorn, Berry,Gorsich, Erin E.,et al. How Does eDNA Compare to Traditional Trapping? Detecting Mosquito Communities in South-African Freshwater Ponds[J],2019,7. |
APA | Krol, Louie,Van der Hoorn, Berry,Gorsich, Erin E.,Trimbos, Krijn,van Bodegom, Peter M.,&Schrama, Maarten.(2019).How Does eDNA Compare to Traditional Trapping? Detecting Mosquito Communities in South-African Freshwater Ponds.FRONTIERS IN ECOLOGY AND EVOLUTION,7. |
MLA | Krol, Louie,et al."How Does eDNA Compare to Traditional Trapping? Detecting Mosquito Communities in South-African Freshwater Ponds".FRONTIERS IN ECOLOGY AND EVOLUTION 7(2019). |
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