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DOI10.1016/j.bbrc.2019.05.052
The CncC/keap1 pathway is activated in high temperature-induced metamorphosis and mediates the expression of Cyp450 genes in silkworm, Bombyx mori
Li, Jinxin1; Mao, Tingting1; Wang, Hui1; Lu, Zhengting1; Qu, Jianwei1; Fang, Yilong1; Chen, Jian1; Li, Mengxue1; Cheng, Xiaoyu1; Hu, Jiahuan1; Gu, Zhiya1; Ni, Min3; Li, Fanchi1,2; Li, Bing1,2
发表日期2019
ISSN0006-291X
EISSN1090-2104
卷号514期号:4页码:1045-1050
英文摘要

Global warming is known to affect the growth, development and reproduction of insects. In this study, the larvae developmental process and endogenous hormone levels under high temperature (36 degrees C) stress were investigated in the lepidopteran model insect Bombyx mori (B. mori). After high temperature treatment, the duration of 5th instar larvae was shortened by 28 degrees 2 h, the content of 20hydroxyecdysone(20E) in hemolymph was significantly increased, and the transcription levels of the 20E response genes E93, Br-C, USP and E75 were up-regulated by 1.35-, 1.25-, 1.28-, and 1.27-fold, respectively. High temperature treatment also elevated the phosphorylation level of Akt and activated the downstream BmCncC/keapl pathway, and the transcription levels of the 20E synthesis-related genes cyp30201, cyp306al, cyp314a1 and cyp315a1 were up-regulated by 1.12-, 1.51-, 2.17- and 1.23-fold, respectively. The transcription levels of cyp302a1 and cyp306a I were significantly decreased in BmN cells after treatment with the double stranded RNA of BmCncC (dsBmCncC), whereas their transcription levels were significantly increased (2.15- and 1.31-fold, respectively) after treatment with the CncC agonist Curcumin. These results demonstrated that high temperature treatment promoted the metamorphosis and the BmCncC/keapl pathway played a role in the metamorphosis of B. mori. Our results provided clues for understanding the CncC/keapl pathway-mediated regulation of metamorphosis of Lepidopteran insects. (C) 2019 Elsevier Inc. All rights reserved.


WOS研究方向Biochemistry & Molecular Biology ; Biophysics
来源期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/100337
作者单位1.Soochow Univ, Sch Basic Med & Biol Sci, Suzhou 215123, Jiangsu, Peoples R China;
2.Soochow Univ, Sericulture Inst, Suzhou 215123, Jiangsu, Peoples R China;
3.Suzhou Univ Sci & Technol, Jiangsu Key Lab Environm Sci & Engn, Suzhou 215009, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Li, Jinxin,Mao, Tingting,Wang, Hui,et al. The CncC/keap1 pathway is activated in high temperature-induced metamorphosis and mediates the expression of Cyp450 genes in silkworm, Bombyx mori[J],2019,514(4):1045-1050.
APA Li, Jinxin.,Mao, Tingting.,Wang, Hui.,Lu, Zhengting.,Qu, Jianwei.,...&Li, Bing.(2019).The CncC/keap1 pathway is activated in high temperature-induced metamorphosis and mediates the expression of Cyp450 genes in silkworm, Bombyx mori.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,514(4),1045-1050.
MLA Li, Jinxin,et al."The CncC/keap1 pathway is activated in high temperature-induced metamorphosis and mediates the expression of Cyp450 genes in silkworm, Bombyx mori".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 514.4(2019):1045-1050.
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